Hydroxyproline-Free Single Composition ABC Collagen Heterotrimer

Formation of collagen hydroxyproline in vitro.

Stetten & Schoenheimer (1943) fed [15N]proline to young rats and showed it to be a good source of the hydroxyproline of the carcass protein. Later, Stetten (1949) found that the efficiency of dietary [15N]hydroxyproline was less than one-twentieth of that of proline as a precursor of collagen hydroxyproline. These findings are of considerable interest since they are contrary to the general obse...

Conversion of Proline to Collagen Hydroxyproline in a Cell-free System from Chick Embryo.

There is a unique relationship between proline hydroxylation and collagen biosynthesis, since proline, rather than free hydroxyproline, appears to be the major precursor of collagen hydroxyproline (1, 2). The early stages of collagen biosynthesis have been shown to be similar to the steps involved in the biosynthesis of other proteins in that hydroxyproline with the highest specific activity ap...

Incorporation of cis-hydroxyproline into protocollagen and collagen. Collagen containing cis-hydroxyproline in place of proline and trans-hydroxyproline is not extruded at a normal rate.

Incubation of cartilage from chick embryos with l*C3,5-cis-hydroxy-DL-proline demonstrated that cis-hydroxyproline is incorporated into the collagen and other protein synthesized by the tissue. Gel filtration of extracts from the tissue demonstrated that the proteins synthesized in the presence of cis-hydroxyproline were about the same size as proteins synthesized under control conditions. Incu...

The role of hydroxyproline in collagen folding: conformational energy calculations on oligopeptides containing proline and hydroxyproline.

We have observed that the rate of folding of the enzymatically hydroxylated form of poly(G1yPro-Pro) into the triple-helical conformation is considerably higher than that of the unhydroxylated polypeptide [R. K. Chopra and V. S. Ananthanarayanan (1982) Proc. Nutl. Acud. Sci. USA 79, 7180-71841. In this study, we examine a plausible kinetic pathway for triple-helix formation by selecting peptide...

The size of the polypeptide precursor of collagen hydroxyproline.